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Article

J Vet Clin 2015; 32(5): 404-409

https://doi.org/10.17555/jvc.2015.10.32.5.404

Published online October 31, 2015

Evaluation for Biocompatibility of Gentamicin-collagen Sponge on the Experimental Animal Wound Model

Sung-Ho Yun1,*, Jihee Park1,*, Jeongkyu Park2,*, Manseop Kim2,*, Dalwon Kim2,*, Moon-Yong Song3,*, Gyoo-Il Kang3,*, Won-Koo Hwang3,*, Sae-Kwang Ku4,*, Kwang-Ho Jang1,*, Young-Sam Kwon1,5,*

*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea
**BNC KOREA, INC., Daegu 704-948, Korea
***Korea Conformity Laboratories, Seoul 137-707, Korea
****College of Oriental Medicine, Daegu Haany University, Daegu 712-715, Korea
*****Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu 702-701, Korea

Copyright © The Korean Society of Veterinary Clinics.

Abstract

The objective of this study was to compare the biocompatibility for local irritation and bioavailability of $Gentacol^{TM}$ and $Collatamp^{TM}$, after single intramuscular loading in rats. Sixty-six male Sprague-Dawley rats were divided into 4 groups; (1) any test materials were not applied into the quadriceps muscles (control group, N = 6), (2) Gentamicin was injected into the quadriceps muscles (Gentamicin group, N = 6), (3) Collatamp was applied into the quadriceps muscles (Collatamp group, N = 27), and (4) Gentacol was applied into the quadriceps muscles (Gentacol group, N = 27). The concentration of gentamicin in muscles was gradually decreased with time after loaded in the both $Gentacol^{TM}$ and $Collatamp^{TM}$ loaded regions. No detectable gentamicin was observed in the plasma of $Gentacol^{TM}$ and $Collatamp^{TM}$ loaded rats. Histologically, moderate to severe inflammatory cell infiltrations including PMN, lymphoid cells and macrophages were observed with slight to moderate edematous changes of muscle fibers after intramuscular injection of gentamicin. However, these histopathological changes of gentamicin injection induced local irritations were dramatically decreases after intramuscular loading of $Collatamp^{TM}$ and $Gentacol^{TM}$. These results suggest $Gentacol^{TM}$ may show favorable local bioavailability and induce only slight local irritations as comparable as $Collatamp^{TM}$ without systemic exposures in the condition of this experiment.

Keywords: Biocompatibility, gentamicin, collagen sponge.

Article

J Vet Clin 2015; 32(5): 404-409

Published online October 31, 2015 https://doi.org/10.17555/jvc.2015.10.32.5.404

Copyright © The Korean Society of Veterinary Clinics.

Evaluation for Biocompatibility of Gentamicin-collagen Sponge on the Experimental Animal Wound Model

Sung-Ho Yun1,*, Jihee Park1,*, Jeongkyu Park2,*, Manseop Kim2,*, Dalwon Kim2,*, Moon-Yong Song3,*, Gyoo-Il Kang3,*, Won-Koo Hwang3,*, Sae-Kwang Ku4,*, Kwang-Ho Jang1,*, Young-Sam Kwon1,5,*

*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea
**BNC KOREA, INC., Daegu 704-948, Korea
***Korea Conformity Laboratories, Seoul 137-707, Korea
****College of Oriental Medicine, Daegu Haany University, Daegu 712-715, Korea
*****Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu 702-701, Korea

Abstract

The objective of this study was to compare the biocompatibility for local irritation and bioavailability of $Gentacol^{TM}$ and $Collatamp^{TM}$, after single intramuscular loading in rats. Sixty-six male Sprague-Dawley rats were divided into 4 groups; (1) any test materials were not applied into the quadriceps muscles (control group, N = 6), (2) Gentamicin was injected into the quadriceps muscles (Gentamicin group, N = 6), (3) Collatamp was applied into the quadriceps muscles (Collatamp group, N = 27), and (4) Gentacol was applied into the quadriceps muscles (Gentacol group, N = 27). The concentration of gentamicin in muscles was gradually decreased with time after loaded in the both $Gentacol^{TM}$ and $Collatamp^{TM}$ loaded regions. No detectable gentamicin was observed in the plasma of $Gentacol^{TM}$ and $Collatamp^{TM}$ loaded rats. Histologically, moderate to severe inflammatory cell infiltrations including PMN, lymphoid cells and macrophages were observed with slight to moderate edematous changes of muscle fibers after intramuscular injection of gentamicin. However, these histopathological changes of gentamicin injection induced local irritations were dramatically decreases after intramuscular loading of $Collatamp^{TM}$ and $Gentacol^{TM}$. These results suggest $Gentacol^{TM}$ may show favorable local bioavailability and induce only slight local irritations as comparable as $Collatamp^{TM}$ without systemic exposures in the condition of this experiment.

Keywords: Biocompatibility, gentamicin, collagen sponge.

Vol.41 No.6 December 2024

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The Korean Society of Veterinary Clinics

pISSN 1598-298X
eISSN 2384-0749

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