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pISSN 1598-298X
eISSN 2384-0749
Ex) Article Title, Author, Keywords
J Vet Clin 2017; 34(4): 261-267
https://doi.org/10.17555/jvc.2017.08.34.4.261
Published online August 30, 2017
Seo Seung Beom1, Sang Yeop Shin1, S. H. S. Dananjaya1, A. B. K. H. De Silva1, Chamilani Nikapitiya2, Jongki Cho1, Gun-Hoo Park3, Chulhong Oh3,4, Do-Hyung Kang3,4, Mahanama De Zoysa1
Copyright © The Korean Society of Veterinary Clinics.
The aim of this study was to investigate the antibacterial properties of chitosan silver nanocomposites (CAgNCs) using pathogenic Vibrio ichthyoenteri as a bacterial model. Results of agar disc diffusion and turbidimetric assays showed that CAgNCs could inhibit the growth of V. ichthyoenteri in concentration dependent manner. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CAgNCs were 75 and 125 μg/mL, respectively. Furthermore, CAgNCs treatment induced the reactive oxygen species (ROS) level in V. ichthyoenteri cells in concentration and time dependent manner, suggesting that it generates oxidative stress, leading to bacterial cell death. The field emission scanning electron microscope (FE-SEM) images of CAgNCs treated V. ichthyoenteri exhibited strong cell membrane damage than un-treated control bacteria. MTT assay results showed the highest cell viability (22%) at 75 μg/mL of CAgNCs treated bacteria samples. The results from this study suggest that CAgNCs is a potential antibacterial agent to control fish pathogenic bacteria.
Keywords: antimicrobial agents, Chitosan-silver nanocomposites, Vibrio ichthyoenteri
J Vet Clin 2017; 34(4): 261-267
Published online August 30, 2017 https://doi.org/10.17555/jvc.2017.08.34.4.261
Copyright © The Korean Society of Veterinary Clinics.
Seo Seung Beom1, Sang Yeop Shin1, S. H. S. Dananjaya1, A. B. K. H. De Silva1, Chamilani Nikapitiya2, Jongki Cho1, Gun-Hoo Park3, Chulhong Oh3,4, Do-Hyung Kang3,4, Mahanama De Zoysa1
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 34134, Republic of Korea
*Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province 63243, Republic of Korea
**Korea Institute of Ocean Science and Technology, Jeju Special Self-Governing Province 63349, Republic of Korea
***Department of Marine Biology, Korea University of Science and Technology, Jeju Special Self-Governing Province 63349, Republic of Korea
The aim of this study was to investigate the antibacterial properties of chitosan silver nanocomposites (CAgNCs) using pathogenic Vibrio ichthyoenteri as a bacterial model. Results of agar disc diffusion and turbidimetric assays showed that CAgNCs could inhibit the growth of V. ichthyoenteri in concentration dependent manner. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CAgNCs were 75 and 125 μg/mL, respectively. Furthermore, CAgNCs treatment induced the reactive oxygen species (ROS) level in V. ichthyoenteri cells in concentration and time dependent manner, suggesting that it generates oxidative stress, leading to bacterial cell death. The field emission scanning electron microscope (FE-SEM) images of CAgNCs treated V. ichthyoenteri exhibited strong cell membrane damage than un-treated control bacteria. MTT assay results showed the highest cell viability (22%) at 75 μg/mL of CAgNCs treated bacteria samples. The results from this study suggest that CAgNCs is a potential antibacterial agent to control fish pathogenic bacteria.
Keywords: antimicrobial agents, Chitosan-silver nanocomposites, Vibrio ichthyoenteri
