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Article

J Vet Clin 2020; 37(1): 23-27

https://doi.org/10.17555/jvc.2020.02.37.1.23

Published online February 29, 2020

Ultra Fast Real-Time PCR for Detection of Babesia gibsoni as Point of Care Test

Yong-Sung Yang2, Myung-Jun Mun3, Young-Min Yun1

College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Korea
*Seogwipo Animal Medical Center, Seogwip 63590, Korea
**CAREVET Co., Ltd., Ansan 15610, Korea

Copyright © The Korean Society of Veterinary Clinics.

Abstract

Between May and November 2018, babesiosis was examined in 162 bloods samples obtained to an animal hospital in Jeju island for anemia and medical examination. Sixty-two of 162 (38.3%) were positive by PCR. The ultra fast real-time PCR test with blood directly analyzed without DNA extraction showed the same results. Accurate diagnosis, treatment and prognosis of babesiosis should be combined with clinical symptoms, blood tests, the babesia antibody test, and the PCR antigen test. Ultra fast real-time PCR, with these tests, is expected to be a point-of-care testing (POCT) for easy, fast and accurate diagnosis of babesiosis in the veterinary clinic.

Keywords: babesia gibsoni, ultra fast real-time PCR, POCT, dog

Article

J Vet Clin 2020; 37(1): 23-27

Published online February 29, 2020 https://doi.org/10.17555/jvc.2020.02.37.1.23

Copyright © The Korean Society of Veterinary Clinics.

Ultra Fast Real-Time PCR for Detection of Babesia gibsoni as Point of Care Test

Yong-Sung Yang2, Myung-Jun Mun3, Young-Min Yun1

College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Korea
*Seogwipo Animal Medical Center, Seogwip 63590, Korea
**CAREVET Co., Ltd., Ansan 15610, Korea

Abstract

Between May and November 2018, babesiosis was examined in 162 bloods samples obtained to an animal hospital in Jeju island for anemia and medical examination. Sixty-two of 162 (38.3%) were positive by PCR. The ultra fast real-time PCR test with blood directly analyzed without DNA extraction showed the same results. Accurate diagnosis, treatment and prognosis of babesiosis should be combined with clinical symptoms, blood tests, the babesia antibody test, and the PCR antigen test. Ultra fast real-time PCR, with these tests, is expected to be a point-of-care testing (POCT) for easy, fast and accurate diagnosis of babesiosis in the veterinary clinic.

Keywords: babesia gibsoni, ultra fast real-time PCR, POCT, dog

Vol.41 No.1 February 2024

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The Korean Society of Veterinary Clinics

pISSN 1598-298X
eISSN 2384-0749

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