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Article

J Vet Clin 2020; 37(5): 242-248

https://doi.org/10.17555/jvc.2020.10.37.5.242

Published online October 31, 2020

Morphological and Molecular Characterization of Apharyngostrigea pipientis Faust, 1918 (Trematoda: Diplostomidea) from Ardeacinerea jouyi, Egretta intermedia and Nycticorax nycticorax in Korea

Hyeon-Cheol Kim1, Eui-Ju Hong2, Si-Yun Ryu2, Kyoung-Seong Choi3, Do-Hyeon Yu4, Jeong-Gon Cho5, Jinho Park5, Joon-Seok Chae6, Bae-Keun Park2

1College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, 2Research Institute of Veterinary Medicine and College of Veterinary Medicine, Chungnam National University, 3College of Ecology and Environmental Science, Kyungpook National University, 4Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, 5College of Veterinary Medicine, Chonbuk National University, 6Laboratory of Veterinary Internal Medicine, Research Institute and BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University

Copyright © The Korean Society of Veterinary Clinics.

Abstract

An intestinal strigeiid trematode, Apharyngostrigea pipientis, was detected from Ardea cinerea jouyi, Egretta intermedia and Nycticorax nycticorax between July, 2006 and June, 2020 in Daejeon metropolitan city and Kangwon-do, Republic of Korea. It was observed with a light and scanning electron microscope (SEM), and molecularly analyzed with 18S rRNA genes. The infection rate of the strigeiid trematode was 25.9% (7/27). The body is cylindrical in whole mount, greatly elongated in outline and shows a strong dorsal curvature. The overall measurements are: length 4.1-4.8 mm; fore-body 1.00-1.20 mm by 0.8-0.9 mm; hind-body 3.2-3.6 mm by 0.43-0.46 mm. The body is covered by a relatively thick cuticle, devoid of spines. The oral sucker leads into a short esophagus that is devoid of a muscular pharynx. The holdfast organ is enormously developed. The vitelline follicles distributed dorsal wall of fore-body and hind-body. This strigeiid trematode especially differs from other species of genus Apharyngostrigea in morphology. Although these A. pipientis are several morphological distinctions from Apharyngostrigea cornu, the partial sequence of 18S rRNA showed the sequence homology of A. pipientis (99.9%) and A. cornu (99.8%), and this result revealed their conspecific relationship between A. cornu and A. pipientis. This is the first report of A. pipientis in the Republic of Korea.

Keywords: Apharyngostrigea pipientis, Ardea cinerea jouyi, Egretta intermedia, Nycticorax nycticorax, Korea

Article

J Vet Clin 2020; 37(5): 242-248

Published online October 31, 2020 https://doi.org/10.17555/jvc.2020.10.37.5.242

Copyright © The Korean Society of Veterinary Clinics.

Morphological and Molecular Characterization of Apharyngostrigea pipientis Faust, 1918 (Trematoda: Diplostomidea) from Ardeacinerea jouyi, Egretta intermedia and Nycticorax nycticorax in Korea

Hyeon-Cheol Kim1, Eui-Ju Hong2, Si-Yun Ryu2, Kyoung-Seong Choi3, Do-Hyeon Yu4, Jeong-Gon Cho5, Jinho Park5, Joon-Seok Chae6, Bae-Keun Park2

1College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, 2Research Institute of Veterinary Medicine and College of Veterinary Medicine, Chungnam National University, 3College of Ecology and Environmental Science, Kyungpook National University, 4Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, 5College of Veterinary Medicine, Chonbuk National University, 6Laboratory of Veterinary Internal Medicine, Research Institute and BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University

Abstract

An intestinal strigeiid trematode, Apharyngostrigea pipientis, was detected from Ardea cinerea jouyi, Egretta intermedia and Nycticorax nycticorax between July, 2006 and June, 2020 in Daejeon metropolitan city and Kangwon-do, Republic of Korea. It was observed with a light and scanning electron microscope (SEM), and molecularly analyzed with 18S rRNA genes. The infection rate of the strigeiid trematode was 25.9% (7/27). The body is cylindrical in whole mount, greatly elongated in outline and shows a strong dorsal curvature. The overall measurements are: length 4.1-4.8 mm; fore-body 1.00-1.20 mm by 0.8-0.9 mm; hind-body 3.2-3.6 mm by 0.43-0.46 mm. The body is covered by a relatively thick cuticle, devoid of spines. The oral sucker leads into a short esophagus that is devoid of a muscular pharynx. The holdfast organ is enormously developed. The vitelline follicles distributed dorsal wall of fore-body and hind-body. This strigeiid trematode especially differs from other species of genus Apharyngostrigea in morphology. Although these A. pipientis are several morphological distinctions from Apharyngostrigea cornu, the partial sequence of 18S rRNA showed the sequence homology of A. pipientis (99.9%) and A. cornu (99.8%), and this result revealed their conspecific relationship between A. cornu and A. pipientis. This is the first report of A. pipientis in the Republic of Korea.

Keywords: Apharyngostrigea pipientis, Ardea cinerea jouyi, Egretta intermedia, Nycticorax nycticorax, Korea